| 不同浓度IL-17A中和性抗体对CIA小鼠血清中炎症细胞因子表达的影响* |
| Effects of different concentrations of IL-17A neutralizing antibodies to the expression of inflammatory cytokines in the serum of CIA mice. |
| |
| DOI： |
| 中文关键词: IL-17A 中和性抗体 CIA小鼠 细胞因子 实验动物 |
| 英文关键词:IL-17A Neutralizing antibody CIA mice Cytokines |
| 基金项目: |
|
| 通讯作者:刘平1 郭锦荣2* 姚弘毅1 韦卓1 1华中科技大学同济医学院附属梨园医院，湖北 武汉 430077 ；2.广西中医药大学，广西南宁530001 |
| 摘要点击次数: 5866 |
| 全文下载次数: 0 |
| 中文摘要: |
| [摘要]目的 通过建立胶原诱导关节炎（CIA）小鼠模型，探讨不同浓度的IL-17A中和性抗体对CIA小鼠血清中炎症细胞因子表达。方法 将48只SPF级C57BL/6雄性小鼠随机分为4组：A（空白对照）组、B（CIA+生理盐水）组、C（CIA+高浓度）组、D（CIA+低浓度）组；采用鸡II型胶原加氟氏完全佐剂乳化制备抗原尾部多点皮下注射；初次免疫2周后以同样方法加强免疫，诱导CIA小鼠模型出现后在小鼠腹腔注射细胞因子中和性抗体或生理盐水，观察2周并记录小鼠关节及关节外表现，检测血清TNF-a、IL-17、IL-6表达水平。结果C、D与B比较TNF-a、IL-17、IL-6表达水平显著降低，并且IL-17A中和性抗体高浓度组较低浓度组血清中炎症细胞因子表达水平降低，差异有统计学意义（P<0.05）。结论IL-17A中和性抗体可显著降低CIA小鼠模型血清中炎症细胞因子TNF-a、IL-17、IL-6表达水平，且以高浓度组较低浓度组改善骨关节功能更显著，其作用机制可能是通过调节Th17细胞参与介导的细胞因子-破骨细胞损伤免疫机制，抑制炎症细胞因子表达，进一步影响破骨细胞因子来实现。 |
| 英文摘要: |
| [Abstract] Objective The establishment of collagen induced arthritis model, Discussion different concentrations of IL-17A neutralizing antibody to CIA serum inflammatory cytokines in mice. Methods 48 male SPF C57BL/6 mice were randomly divided into 4 groups: A（Normal group）group; B（The CIA and Saline Group）group; C（The CIA and high concentration group）group; D（The CIA and low concentration group）group. Use of chicken type II collagen fluorine's complete adjuvant emulsion preparation of antigen tail more subcutaneous injection; Two weeks later, Primary immune with the same method for the second immunization, intraperitoneal inject neutralizing anti -murine interleukin-17 antibody or physiological saline after CIA mice models were induced; persistent observation and data record of the change of the general situation, mice weight，arthritis index and thickness of fat in two weeks, testing the CIA mice serum TNF-a, IL-6, IL-17 expression level. Results C, D and B compared of TNF-a, IL-17 and IL-6 expression level decreased significantly; neutralizing anti-murine interleukin-17 antibody of the high concentration group were lower than low concentrations in serum inflammatory cytokine expression level, the difference was statistically significant （P <0.05）. Conclusion Neutralizes interleukin-17 antibody can significantly reduce Inflammatory cytokine of the CIA mice model in serum TNF-a, IL-17, IL-6 expression level, And the highest concentration group improved joint function more significant than the low concentration group, The mechanism may be involved in regulating Th17 mediated by cytokines-osteoclasts immune system, Inhibit the inflammatory cytokine expression, Further affect osteoclast factor to achieve. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
