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纳米多孔β-磷酸三钙复合自体骨折血肿的异位成骨潜能的初步实验研究
A preliminary experimental study on the ectopic osteogenesis potential of nano-porous β-tricalcium phosphate composite autologous fracture hematoma
投稿时间:2023-08-23  
DOI:10.3969/j.issn.1672-5972.2024.03.002
中文关键词:  骨折血肿  β-磷酸三钙  骨再生  骨缺损  组织工程
英文关键词:Fracture hematoma  β-tricalcium phosphate  Bone regeneration  Bone defect  Tissue engineering
基金项目:
作者单位邮编
刘豪 湖南师范大学附属岳阳医院骨科湖南 岳阳414000 414000
杨佩 西安交通大学第二附属医院骨科陕西 西安710006 710006
王春生 西安交通大学第二附属医院骨科陕西 西安710006 710006
张旭 湖南师范大学附属岳阳医院骨科湖南 岳阳414000 414000
刘琦 湖南师范大学附属岳阳医院骨科湖南 岳阳414000 414000
刘芳 湖南师范大学附属岳阳医院骨科湖南 岳阳414000 414000
熊执政* 湖南师范大学附属岳阳医院骨科湖南 岳阳414000 414000
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中文摘要:
      目的 探究纳米多孔β-磷酸三钙(β-tricalcium phosphate, β-TCP)复合自体骨折血肿的异位成骨潜能。方法 将78只新西兰大白兔随机分为A、B、C、D和E共5组,髂骨翼上截取约5 mm×10 mm×10 mm的骨块,造成人工骨折并形成血肿,然后分别将血肿/β-TCP、β-TCP、血肿/髂骨、髂骨种植于背阔肌下,于模型建立后第1、4和8周取下背阔肌下标本,采用病理染色技术和免疫组织化学技术评估新骨生成、支架材料降解情况及进行生物力学测试。E组用于观察血肿在β-TCP支架内部的吸附观察,E组动物健侧髂骨设为正常对照组(F组),仅行生物力学测试。结果 髂骨骨折后第4天大量的细胞和细胞外基质充填在支架内,此时的血肿/β-TCP机械性能稍优于单纯β-TCP支架。A、C两组标本有周围有较多的新生骨组织及血管组织,而B、D两组标本却只能见到极少量散在的类骨质形成,且D组标本吸收明显;在残余支架体积方面,A、B两组之间差异无统计学意义(P>0.05)。A、C两组新生骨量随着时间的延长而增加(P均<0.01),而B、D两组新生骨量随时间的变化差异无统计学意义(P>0.05)。结论 β-TCP与骨折血肿内活性成分具有良好的相容性,β-TCP复合骨折血肿后具有确切的成骨潜能,有望作为一种新型的陈旧性骨折及骨缺损的治疗策略。
英文摘要:
      Objective To investigate the ectopic osteogenesis potential of nano-porous β-tricalcium phosphate complex autogenous fracture hematoma.Methods Seventy-eight New Zealand white rabbits were randomly divided into 5 groups: Group A, B, C, D and E. Bone fragments of approximately 5 mm×10 mm×10 mm were extracted from the upper part of the iliac bone to create an artificial fracture and form hematoma, then hematoma/β-TCP, β-TCP, hematoma/iliac crest and iliac crest were implanted under latissimus dorsal muscle, and sublatissimus dorsal muscle specimens were removed at 1, 4 and 8 week after the establishment of the model. New bone formation, degradation of scaffold materials and biomechanical tests were evaluated by pathological staining and immunohistochemistry. Group E was used to observe the hematoma while observing the adsorption inside the β-TCP stent. The healthy iliac bone of group E animals was set as a normal control (group F), and only biomechanical tests were performed.Results A large number of cells and extracellular matrix were filled in the scaffold on day 4 after iliac fracture, and the mechanical properties of hematoma/β-TCP were slightly better than that of β-TCP scaffold alone. There were more new bone tissue and vascular tissue around the specimens of group A and group C, while only a small amount of scattered osteoid formation could be seen in the specimens of group B and group D, and the absorption of specimens of group D was obvious. There was no significant difference in the residual scaffold volume between group A and group B (P>0.05). The new bone mass of group A and group C increased with the extension of time (P<0.01), but the change of new bone mass of group B and group D had no statistical significance with the extension of time (P>0.05).Conclusion β-TCP has good compatibility with the active ingredients in fracture hematoma, and β-TCP has definite osteogenic potential after fracture hematoma, which is expected to be a new treatment strategy for old fractures and bone defects.
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