| Objective To investigate the ectopic osteogenesis potential of nano-porous β-tricalcium phosphate complex autogenous fracture hematoma.Methods Seventy-eight New Zealand white rabbits were randomly divided into 5 groups: Group A, B, C, D and E. Bone fragments of approximately 5 mm×10 mm×10 mm were extracted from the upper part of the iliac bone to create an artificial fracture and form hematoma, then hematoma/β-TCP, β-TCP, hematoma/iliac crest and iliac crest were implanted under latissimus dorsal muscle, and sublatissimus dorsal muscle specimens were removed at 1, 4 and 8 week after the establishment of the model. New bone formation, degradation of scaffold materials and biomechanical tests were evaluated by pathological staining and immunohistochemistry. Group E was used to observe the hematoma while observing the adsorption inside the β-TCP stent. The healthy iliac bone of group E animals was set as a normal control (group F), and only biomechanical tests were performed.Results A large number of cells and extracellular matrix were filled in the scaffold on day 4 after iliac fracture, and the mechanical properties of hematoma/β-TCP were slightly better than that of β-TCP scaffold alone. There were more new bone tissue and vascular tissue around the specimens of group A and group C, while only a small amount of scattered osteoid formation could be seen in the specimens of group B and group D, and the absorption of specimens of group D was obvious. There was no significant difference in the residual scaffold volume between group A and group B (P>0.05). The new bone mass of group A and group C increased with the extension of time (P<0.01), but the change of new bone mass of group B and group D had no statistical significance with the extension of time (P>0.05).Conclusion β-TCP has good compatibility with the active ingredients in fracture hematoma, and β-TCP has definite osteogenic potential after fracture hematoma, which is expected to be a new treatment strategy for old fractures and bone defects. |
