Objective To explore the role of HIF-3α in the invasion and metastasis of osteosarcoma, and observe the effect of hypoxia on the invasion ability of osteosarcoma MG-63 cells. Methods Collect 15 cases of osteosarcoma tissues and 15 cases of osteochondroma tissues, and detect the expression of HIF-3α in osteosarcoma tissues and osteochondroma tissues by immunohistochemical staining; MG-63 cells were stimulated by hypoxia and quantitative RT-PCR and Western Blot were used to detect the expression of HIF-3α in MG-63 cells; cell adhesion test, cell migration test and cell invasion test were used to detect the adhesion, migration and invasion ability of MG-63 cells. In MG-63 cells, after knocking down HIF-3α or overexpressing HIF-3α, the effect of hypoxia on cell adhesion, migration, and invasion ability was detected. Results The results of immunohistochemistry showed that HIF-3α was highly expressed in osteosarcoma tissues and HIF-3α was low expressed in osteochondroma tissues. Compared with normoxic conditions, hypoxia promoted the expression of HIF-3α mRNA and HIF-3α protein in MG-63 cells, and hypoxia enhanced the adhesion, migration, and invasion capabilities of MG-63 cells. The knockdown of HIF-3α in MG-63 cells weakened the adhesion, migration, and invasion ability of MG-63 cells, while the overexpression of HIF-3α enhanced the adhesion, migration, and invasion ability of MG-63 cells. Conclusion Hypoxia promotes the expression of HIF-3α in MG-63 cells and enhances the invasion and metastasis ability of MG-63 cells. HIF-3α plays an important role in the invasion and metastasis of MG-63 cells induced by hypoxia. |