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HIF-3α在骨肉瘤组织中的表达及低氧状态对MG-63细胞侵袭能力的影响
The expression of HIF-3α in osteosarcoma tissues and the effect of hypoxia on the invasion ability of MG-63 cells
  
DOI:
中文关键词:  骨肉瘤  低氧诱导因子3α  侵袭转移  MG-63
英文关键词:Osteosarcoma  Hypoxia-inducible factor 3α  Invasion and metastasis  MG-63
基金项目:
作者单位
盛奇智 阮锋 魏优秀 李广磊 刘平*  
通讯作者:盛奇智 阮锋 魏优秀 李广磊 刘平*    
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中文摘要:
      目的 探讨HIF-3α在骨肉瘤侵袭转移中的作用,观察低氧对骨肉瘤MG-63细胞侵袭能力的影响。方法 收集15例骨肉瘤组织和15例骨软骨瘤组织,用免疫组织化学染色方法检测骨肉瘤组织和骨软骨瘤组织中HIF-3α的表达情况;MG-63细胞低氧刺激,用定量RT-PCR和Western Blot方法检测MG-63细胞中HIF-3α的表达情况;用细胞黏附实验、细胞迁移实验及细胞侵袭实验检测MG-63细胞的黏附、迁移及侵袭能力。在MG-63细胞中,敲降HIF-3α或过表达HIF-3α后,检测低氧对细胞的黏附、迁移及侵袭能力的影响。结果 免疫组化结果显示骨肉瘤组织中HIF-3α高表达而骨软骨瘤组织中HIF-3α低表达。与常氧条件相比,低氧促进MG-63细胞中HIF-3α mRNA和HIF-3α蛋白的表达,低氧增强MG-63细胞的黏附、迁移及侵袭能力。MG-63细胞中HIF-3α的敲降减弱了MG-63细胞的黏附、迁移及侵袭能力而HIF-3α的过表达增强了MG-63细胞的黏附、迁移及侵袭能力。结论 低氧促进了MG-63细胞中的HIF-3α的表达,增强了MG-63细胞的侵袭转移能力,HIF-3α在低氧诱导的MG-63细胞侵袭转移中具有重要作用。
英文摘要:
      Objective To explore the role of HIF-3α in the invasion and metastasis of osteosarcoma, and observe the effect of hypoxia on the invasion ability of osteosarcoma MG-63 cells. Methods Collect 15 cases of osteosarcoma tissues and 15 cases of osteochondroma tissues, and detect the expression of HIF-3α in osteosarcoma tissues and osteochondroma tissues by immunohistochemical staining; MG-63 cells were stimulated by hypoxia and quantitative RT-PCR and Western Blot were used to detect the expression of HIF-3α in MG-63 cells; cell adhesion test, cell migration test and cell invasion test were used to detect the adhesion, migration and invasion ability of MG-63 cells. In MG-63 cells, after knocking down HIF-3α or overexpressing HIF-3α, the effect of hypoxia on cell adhesion, migration, and invasion ability was detected. Results The results of immunohistochemistry showed that HIF-3α was highly expressed in osteosarcoma tissues and HIF-3α was low expressed in osteochondroma tissues. Compared with normoxic conditions, hypoxia promoted the expression of HIF-3α mRNA and HIF-3α protein in MG-63 cells, and hypoxia enhanced the adhesion, migration, and invasion capabilities of MG-63 cells. The knockdown of HIF-3α in MG-63 cells weakened the adhesion, migration, and invasion ability of MG-63 cells, while the overexpression of HIF-3α enhanced the adhesion, migration, and invasion ability of MG-63 cells. Conclusion Hypoxia promotes the expression of HIF-3α in MG-63 cells and enhances the invasion and metastasis ability of MG-63 cells. HIF-3α plays an important role in the invasion and metastasis of MG-63 cells induced by hypoxia.
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