| Objective To investigate the mechanism of L-Mimosine induced cell apoptosis in osteosarcoma cell lines. Methods Osteosarcoma cell lines MG-63 and U2-OS were treated with 0 μM, 100 μM, 200 μM, 400 μM L-Mimosine, and 400 μM L-Mimosine is added with 100mg/mL FAC. CCK-8 was used to test cell proliferation, FITC-Annexin V-PI was carried out to investigate cell apoptosis, Hoechst staining was used to investigate nuclear pyknosis, and western blot was executed to detect the DNA damage and repair related protein expression level. Results CCK-8 results showed that the 400 μM L-Mimosine was used to treat MG-63 and U2-OS cell lines, the cell inhibition rates were 81.1%±1.6 and 76.7%±2.1%, respectively. Flow cytometry test showed that, after 48 hours treatment, MG-63 and U2-OS cells apoptosis rates were 54.1%±12.6% and 46.2%±14.7%, nuclear pyknosis rates were 53.8%±10.6% and 49.2%+8.3%. In addition, FAC can attenuate the effect of L-Mimosine that increasing cell inhibition and apoptosis rate. Western blot showed that the protein expression levels of key regulators involved in DNA damage and repair process were changed significantly after treated with L-Mimosine and FAC. Conclusion L-Mimosine can effectively inhibit the proliferation of osteosarcoma cells via inducing the cell apoptosis process, and the molecular mechanism is related to iron chelation. |
